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FITC(Fluorescein Isothiocyanate,异硫氰酸荧光素)是最常用的荧光探针,最大吸收光波长为490-495 nm,最大发射光波长520-530 nm,呈现明亮的黄绿色荧光。FITC标记山羊抗兔IgG (H+L)抗体为高纯度的抗兔抗体,可特异识别兔IgG重链和轻链,而对其它种属IgG无明显交叉反应,具有本底低、灵敏度高等特点,适用于免疫荧光、流式等检测。
Wu L , Wang X , Zhang J , et al. Flow Cytometric Single-Cell Analysis for Quantitative in Vivo Detection of Protein–Protein Interactions via Relative Reporter Protein Expression Measurement[J]. Analytical Chemistry, 2017, 89(5):2782-2789.