TransDetect® Double-Luciferase Reporter Assay Kit

目录号规格单价
FR201-01-V2 50 rxns530
FR201-02-V2 200 rxns1800
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产品详情介绍

萤火虫荧光素酶 (firefly luciferase)和海肾萤光素酶(Renilla luciferase)可分别催化荧光素 (luciferin)或腔肠素 (coelenterazine) 氧化形成oxyluciferin或coelenteramide ,并在此过程中产生生物荧光。TransDetect® Double-Luciferase Reporter Assay Kit首先以荧光素为底物检测萤火虫荧光素酶报告基因的活性,之后在淬灭该荧光反应的同时,以腔肠素为底物检测海肾荧光素酶报告基因的活性,具有检测迅速、灵敏度高、检测范围广、无细胞内源活性干扰等特点。


产品组成

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实验数据

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References

1 Ji X, Du Y, Li F, et al. The basic helix‐loop‐helix transcription factor, Os PIL 15, regulates grain size via directly targeting a purine permease gene Os PUP 7 in rice[J]. Plant Biotechnology Journal, 2019.(IF 6.30)

2 Li Y, Feng D, Wang Z, et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion[J]. Cell Death & Differentiation, 2019.(IF 8.00)

3 Liu F, Xu Y, Chang K, et al. The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis[J]. New Phytologist, 2019.(IF 7.43)

4 Qi Y, Ding L, Zhang S, et al. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency[J]. Cell, 2022.(IF 41.58)

5 Cheng Q, Wang J, Li M, et al. CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease[J]. Redox Biology, 2022.(IF 10.78)

6 Di Y, Jing X, Hu K, et al. The c-MYC-WDR43 signalling axis promotes chemoresistance and tumour growth in colorectal cancer by inhibiting p53 activity[J]. Drug Resistance Updates, 2023.(IF 22.84)

7 Jia X, Wang L, Zhao H, et al. The origin and evolution of salicylic acid signaling and biosynthesis in plants[J]. Molecular Plant, 2023.(IF 21.94)

8 Liu Y, Zhang S, Chen L, et al. The molecular mechanism of miR-96-5p in the pathogenesis and treatment of polycystic ovary syndrome[J]. Translational Research, 2023.(IF 10.17)

9 Zhou J, Ge Q, Wang D, et al. Engineering a modular double-transmembrane synthetic receptor system for customizing cellular programs[J]. Cell Reports, 2023.(IF 9.99)

10 Cui Y, Lyu X, Ding L, et al. Global miRNA dosage control of embryonic germ layer specification[J]. Nature, 2021.(IF 42.00)


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