产品详情介绍
TransDetect® Cell Counting Kit，是一种基于水溶性四唑盐的细胞增殖和细胞毒性检测试剂盒。它在电子耦合试剂1-Methoxy PMS存在的情况下，可以被线粒体内的脱氢酶还原为可溶性的橙黄色甲臜(formazan)。甲臜的数量与活细胞的数量成正比。细胞增殖越快、细胞毒性越小、细胞数量越多，则颜色越深，颜色的深浅与细胞数量呈现良好的线性关系。该产品细胞毒性小，对后续实验没有影响，与MTT，XTT，MTS和WST-1相比，此法检测灵敏度更高，线性范围更宽，适用于药物筛选，细胞增殖测定，细胞毒性测定和肿瘤药敏实验。
CCK灵敏度检测
CCK对细胞毒性低
1 Zhao J, Chu H, Zhao Y, et al. A NIR light gated DNA nanodevice for spatiotemporally controlled imaging of microRNA in cells and animals[J]. Journal of the American Chemical Society, 2019.(IF 14.69)
2 Ma M, Cai B, Zhou Z, et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers[J]. Cell Communication and Signaling, 2023.(IF 7.52)
3 Cai B, Ma M, Zhang J, et al. Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development[J]. Cell Death & Disease, 2022.(IF 8.46)
4 Zhong F, Liu J, Gao C, et al. Downstream regulatory network of MYBL2 mediating its oncogenic role in melanoma[J]. Frontiers in Oncology, 2022.(IF 6.24)
5 Zhao M M, Zhu Y, Zhang L, et al. Novel cleavage sites identified in SARS-CoV-2 spike protein reveal mechanism for cathepsin L-facilitated viral infection and treatment strategies[J]. Cell Discovery, 2022.(IF 38.07)
6 Liao N, Su L, Zheng Y, et al. In vivo tracking of cell viability for adoptive natural killer cell‐based immunotherapy by ratiometric NIR‐II fluorescence imaging[J]. Angewandte Chemie, 2021.(IF 15.33)
7 Liao N, Su L, Cao Y, et al. Tracking cell viability for adipose-derived mesenchymal stem cell-based therapy by quantitative fluorescence imaging in the second near-infrared window[J]. ACS nano, 2022.(IF 15.88)
