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pEASY®-Blunt Zero Cloning Kit

Blunt Zero基因克隆试剂盒(双抗性、零背景、无蓝白斑筛选)

目录号: CB501-01

单 价:¥780

规格:
20 rxns
60 rxns
数量:
-
+
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产品详情介绍

该载体通过自杀基因表达与否筛选阳性重组子。当载体与片段连接成功时,自杀基因无法正确表达,包含重组子的细胞可以正常生长;当载体与片段连接不成功时,自杀基因正确表达,包含载体的细胞无法生长,即“Zero”背景。适用于平端克隆。



产品组成

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References

1 Lin J L, Fang X, Li J X, et al. Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds[J]. Nature Plants, 2023.(IF 17.35)

2 Luo Y, Liu S, Xue J, et al. High-throughput screening of spike variants uncovers the key residues that alter the affinity and antigenicity of SARS-CoV-2[J]. Cell Discovery, 2023.(IF 38.07)

3 Wen R, Wang K, Zan X. Characterization of two full-length tubuliform silk gene sequences from Neoscona theisi reveals intragenic concerted evolution and multiple copies in genome[J]. International Journal of Biological Macromolecules, 2022.(IF 8.00)

4 Luo C, Yan J, Liu W, et al. Genetic mapping and genome-wide association study identify BhYAB4 as the candidate gene regulating seed shape in wax gourd (Benincasa hispida)[J]. Frontiers in Plant Science, 2022.(IF 6.62)

5 Chen J, Hu X, Shi T, et al. Metabolite‐based genome‐wide association study enables dissection of the flavonoid decoration pathway of wheat kernels[J]. Plant Biotechnology Journal, 2020.(IF 6.84)


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